Part:BBa_K4841011:Design
CI857/PR（T41C）
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cI857/pR promoter, which requires cell growth at 288C, heat induction at 388C for 2 h, and further 6 h post-heat induction to reach its highest lysis efficiency (96.3+0.4%), this autolytic vector broadens the growth temperature and shortens the time required for maximal cell lysis, and thus complements and improves the previous low temperature one.
Source
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References
1. Jechlinger, W., Szostak, M. P., Witte, A., & Lubitz, W. (1999). Altered temperature induction sensitivity of the lambda pR/cI857 system for controlled gene E expression in Escherichia coli. FEMS microbiology letters, 173(2), 347–352.
2. Cai, Z., Xu, W., Xue, R., & Lin, Z. (2008). Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening. Protein engineering, design & selection : PEDS, 21(11), 681–687.